Anti-GLUD1 Antibody Picoband® APC-vervoeging

GLUD1 (glutamate dehydrogenase 1) is a mitochondrial matrix enzyme, one of the family of glutamate dehydrogenases that are ubiquitous in life, with a key role in nitrogen and glutamate (Glu) metabolism and energy homeostasis. This dehydrogenase is expressed at high levels in liver, brain, pancreas and kidney, but not in muscle. In the pancreatic cells, GLUD1 is thought to be involved in insulin secretion mechanisms. In nervous tissue, where glutamate is present in concentrations higher than in the other tissues, GLUD1 appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification.

Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog; RAD21; HR21, KIAA0078, NXP1

GLUD1

2746

P00367

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human GLUD1 recombinant protein (Position: A210-T558) .

Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. .

Flow Cytometry

Cancer, Cell Biology, Cell Cycle, Cell Division,2339, Chromosome Structure, DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling

Immunogen affinity purified.

Liquid

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. .

1. Smith TJ, Schmidt T, Fang J, Wu J, Siuzdak G, Stanley CA (May 2002) . The structure of apo human glutamate dehydrogenase details subunit communication and allostery. J. Mol. Biol. 318 (3) : 765–77. 2. George A, Bell JE (December 1980) . Effects of adenosine 5'-diphosphate on bovine glutamate dehydrogenase: diethyl pyrocarbonate modification. Biochemistry. 19 (26) : 6057–61. Glaser B, Blech I, Krakinovsky Y, Ekstein J, Gillis D, Mazor-Aronovitch K, Landau H, Abeliovich D (Oct 2011) . ABCC8 mutation allele frequency in the Ashkenazi Jewish population and risk of focal hyperinsulinemic hypoglycemia. Genetics in Medicine. 13 (10) : 891–4.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

61 kDa

6

Double-strand-break repair protein rad21 homolog

Nucleus . Chromosome . Chromosome, centromere . Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis. .

Lysosome-associated membrane glycoprotein 2 (Lamp2)

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.