Anti-GM130 GOLGA2 Konijn Monoklonale Antilichaam

Boster Bio Anti-GM130 GOLGA2 Rabbit Monoclonal Antibody catalog # M05865. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Golgin subfamily A member 2;130 kDa cis-Golgi matrix protein ; GM130 ; GM130 autoantigen ; Golgin-95 ; GOLGA2

GOLGA2

2801

Q08379

Human, Mouse, Rat

A synthesized peptide derived from human GM130

Clone: AHB-7

Expressed in all tissues examined.

WB, IHC, ICC, IF, IP

Golgi Proteins, Organelles, Protein Trafficking, Signal Transduction, Subcellular Markers

Affinity-chromatography

0.5mg/ml

Liquid

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity) . Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940) . Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin- alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940) . Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity) . Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421) . Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401) . .

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=M05865

113086 MW

150 kDa

Golgin subfamily A member 2

Golgi apparatus, cis-Golgi network membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, spindle pole . Peripheral membrane protein associated with cis-Golgi stacks (By similarity) . Associates with the mitotic spindle during mitosis (PubMed:26165940) . .

T-cell surface glycoprotein CD3 zeta chain

Rabbit IgG

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.