Anti-Golimumab ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Golimumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Golimumab will be captured by immobilized Golimumab. After washing away any unbound substances, a biotin-labeled Golimumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Golimumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Golimumab

CNTO 148, CAS: 476181-74-5

Colorimetric

Quantitative

Plasma, Serum

156.25 - 10,000 ng/mL

80-120%

85.1 ng/mL

2-8 °C

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.

For Research Use Only.