Anti-Groen fluorescerend eiwit (GFP) Antilichaam

The green fluorescent protein (GFP) is a 27 kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395 nm and emission maximum at 509 nm, which is similar to that of fluorescein. GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell. This GFP rabbit antibody was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization (1) . This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and co-workers. The antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.

Our Anti-Green fluorescent protein (GFP) rabbit polyclonal primary antibody detects species independent Green fluorescent protein (GFP), and is affinity purified. It is validated for use in IHC, WB.

Our Anti-Green fluorescent protein (GFP) rabbit polyclonal primary antibody detects Green fluorescent protein (GFP), and is affinity purified. It is validated for use in IHC, WB.

Aequorea victoria green fluorescent protein

Q6YGZ0

Species Independent

Recombinant AcGFP purified from E. coli

Green fluorescent protein (GFP)

Unconjugated

IHC, WB

Affinity purified

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

27 kDa

For research use only.

25°C (ambient)

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) . Avoid repetitive freeze/thaw cycles.

1:2000-1:5000

1:1000-1:5000

GFP, cross reactivity with other mutant forms is expected as immunogen was a whole molecule GFP.

Rabbit

Lyophilized

IgG

Jellyfish