Anti-GRPEL1 Antibody Picoband®

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins.

Boster Bio Anti-GRPEL1 Antibody Picoband® catalog # A12830-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Mediator of RNA polymerase II transcription subunit 9; Mediator complex subunit 9; MED9; MED25

GRPEL1

80273

Q9HAV7

Human

No cross-reactivity with other proteins.

E.coli-derived human GRPEL1 recombinant protein (Position: Q90-A217) .

Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Epigenetics and Nuclear Signaling, Mediator Complex, Pol II Transcription, Polymerase Associated Factors, RNA Polymerase, Transcription

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

1. Choglay, A. A., Chapple, J. P., Blatch, G. L., Cheetham, M. E. Identification and characterization of a human mitochondrial homologue of the bacterial co-chaperone GrpE. Gene 267: 125-134, 2001. 2. Konovalova, S., Liu, X., Manjunath, P., Baral, S., Neupane, N., Hilander, T., Yang, Y., Balboa, D., Terzioglu, M., Euro, L., Varjosalo, M., Tyynismaa, H. Redox regulation of GRPEL2 nucleotide exchange factor for mitochondrial HSP70 chaperone. Redox Biol. 19: 37-45, 2018. 3. Naylor, D. J., Stines, A. P., Hoogenraad, N. J., Hoj, P. B. Evidence for the existence of distinct mammalian cytosolic, microsomal, and two mitochondrial GrpE-like proteins, the co-chaperones of specific Hsp70 members. J. Biol. Chem. 273: 21169-21177, 1998.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

https://www.bosterbio.com/msds?sku=A12830-1

24 kDa

6

Mediator complex subunit 9

Nucleus .

Rabbit IgG

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.