Anti-HICE1 HAUS8 Antilichaam

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the gamma TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.

Boster Bio Anti-HICE1 HAUS8 Antibody (Catalog # A12994) . Tested in ELISA, WB applications. This antibody reacts with Human.

HAUS8, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48, HICE1

HAUS8

Q9BT25

Human

Anti-HICE1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length Hice1 protein.

ELISA, WB

Cancer, Cell Biology, Cell Cycle, Cell Division, Spindle, Tumor Immunology, Tumor-Associated Antigens

This product was adsorbed against GST from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology with the immunizing sequence. Expect reactivity with Hice1 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence. Cross-reactivity with Hice1 from other sources has not been determined.

38 mg/mL by Refractometry

Liquid (sterile filtered)

Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.

Dry Ice Shipping

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

https://www.bosterbio.com/msds?sku=A12994

44857 MW

HAUS augmin-like complex subunit 8

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. During interphase, primarily cytoplasmic and associates with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, additionally associates with the spindle midzone and midbody, respectively.

Antiserum

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide