Anti-His-Tag Antilichaam

The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.

Our Anti-His-Tag rabbit polyclonal primary antibody detects species independent His-Tag , and is affinity-purified. It is validated for use in ELISA, ICC, IHC-Frozen, WB.

Our Anti-His-Tag rabbit polyclonal primary antibody detects His-Tag, and is affinity-purified. It is validated for use in ELISA, ICC, IHC-Frozen, WB.

Species Independent

The antiserum was produced by immunizing rabbit with synthesized peptide containing 6 Histidine residues.

His-Tag

Unconjugated

ELISA, ICC, IHC-Frozen, WB

Affinity-purified

PBS, pH 7.4 with 0.02% sodium azide.

For research use only.

2-8°C (on cold packs)

Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.

5 μg/mL

1:500-1:1000

5 μg/sample

His-tagged fusion proteins.

5 μg/mL

Rabbit

Liquid

IgG

Species Independent