Anti-histidine-tag (Zijn) Antilichaam

The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.

Our Anti-Histidine-tag (His) mouse monoclonal primary antibody detects species independent Histidine-tag (His), and is igg. It is validated for use in ICC, WB.

Our Anti-Histidine-tag (His) mouse monoclonal primary antibody detects Histidine-tag (His), and is IgG. It is validated for use in ICC, WB.

Species Independent

Synthetic peptide containing 6 Histidine residues.

Histidine-tag (His)

HIS.H8

Unconjugated

ICC, WB

IgG

PBS, pH 7.4 with 0.05% sodium azide

For research use only.

2-8°C (on cold packs)

Stable for 1 year at -20°C unopened. After opening we recommend aliquoting and storing at -20°C for up to 6 months. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap.

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His-tagged fusion proteins.

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Mouse

Liquid

IgG2b

Species Independent