Anti-histone deacetylase 5 HDAC5 Antibody Picoband® fluoro488 Vervoeging

Histone deacetylase 5, also called HDAC5 or KIAA0600, is an enzyme that in humans is encodes by the HDAC5 gene. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. This gene is mapped to 17q21.31. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is though to be associated with colon cancer.

Histone deacetylase 5; HD5;3.5.1.98; Antigen NY-CO-9; HDAC5; KIAA0600

HDAC5

10014

Q9UQL6

Rat, Mouse, Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human HDAC5, different from the related rat and mouse sequences by two amino acids.

Ubiquitous.

Flow Cytometry

Acetylation, Cardiovascular, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Hypertrophy, Signaling Pathways, Stem Cells

Immunogen affinity purified.

Liquid

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) . Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. .

1. McKinsey, T. A., Zhang, C.-L., Lu, J., Olson, E. N. Signal-dependent nuclear export of a histone deacetylase regulates muscle differentiation. Nature 408: 106-111, 2000. 2. Potthoff, M. J., Wu, H., Arnold, M. A., Shelton, J. M., Backs, J., McAnally, J., Richardson, J. A., Bassel-Duby, R., Olson, E. N. Histone deacetylase degradation and MEF2 activation promote the formation of slow-twitch myofibers. J. Clin. Invest. 117: 2459-2467, 2007.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

121978 MW

No cross reactivity with other proteins.

6

Histone deacetylase 5

Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.

Belongs to the histone deacetylase family. HD type 2 subfamily.

Histone deacetylase 5

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.