Anti-HRSV F/Fusion glycoproteïne F0 Human IgG ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant HRSV F/Fusion glycoprotein F0 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-HRSV F/Fusion glycoprotein F0 Human IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-HRSV F/Fusion glycoprotein F0 Human IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Fusion glycoprotein F0

Colorimetric

Quantitative

Plasma, Serum

1.56 ng/mL - 100 ng/mL

80-120%

0.88 ng/mL

2-8 °C

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.

For Research Use Only.