Anti-HSD11B2 Antibody Picoband® fluoro488 Vervoeging

Corticosteroid 11-β-dehydrogenase isozyme 2, also known as11-β-hydroxysteroid dehydrogenase 2, is an enzymethat in humans is encoded by theHSD11B2gene. There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.

Targeting protein for Xklp2; Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; Protein fls353; Restricted expression proliferation-associated protein 100; p100; TPX2; C20orf1; C20orf2; DIL2; HCA519

HSD11B2

3291

P80365

Human

No cross-reactivity with other proteins.

E.coli-derived human HSD11B2 recombinant protein (Position: K96-Q377) .

Expressed in lung carcinoma cell lines but not in normal lung tissues.

Flow Cytometry

Cancer, Cell Biology, Cell Cycle, Cell Division, Chromosome Structure, Epigenetics and Nuclear Signaling, Spindle

Immunogen affinity purified.

Liquid

Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764) . Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764) . TPX2 is inactivated upon binding to importin- alpha (PubMed:26165940) . At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940) .

1. Albiston AL, Obeyesekere VR, Smith RE, Krozowski ZS (November 1994) . Cloning and tissue distribution of the human 11 beta-hydroxysteroid dehydrogenase type 2 enzyme. Mol. Cell. Endocrinol. 105 (2) : R11–7. 2. Brown RW, Chapman KE, Kotelevtsev Y, Yau JL, Lindsay RS, Brett L, Leckie C, Murad P, Lyons V, Mullins JJ, Edwards CR, Seckl JR (February 1996) . Cloning and production of antisera to human placental 11 beta-hydroxysteroid dehydrogenase type 2. Biochem. J. 313 (Pt 3) (Pt 3) : 1007–17.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

TPX2, microtubule nucleation factor

Nucleus.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.