Anti-Hsp60 (N-terminaal gebied) Antilichaam

Heat shock proteins (Hsp) are a family of highly conserved proteins that include both constitutively expressed (Hsp60, Hsp70, and Hsp90) and stress-induced (Hsp27 and Hsp72) proteins. Hsp60 is a mitochondrial protein that promotes protein folding and facilitates proteolytic degradation of misfolded or denatured proteins in the mitochondria. Hsp10 interacts with Hsp60 to regulate its substrate binding and ATPase activity. In HeLa and Jurkat mitochondria, Hsp60 associates with caspase-3 to form a complex that dissociates and releases from the mitochondria during apoptosis. Hsp60 accelerates the maturation of procaspase-3 through its ATP-dependent “foldase” activity. In addition to its protein folding activity, Hsp60 can bind the toll-like receptor-4 complex leading to production of TNFα and stimulation of a pro-inflammatory response in macrophages. Thus, the protein folding function of Hsp60 is involved in protein folding in both normal and apoptotic cells, while release of Hsp60 during necrosis is thought to stimulate a pro-inflammatory response.

Our Hsp60 (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Hsp60 (N-terminal region) and is protein a purified. It is great for use in ELISA, WB, ICC.

Our Hsp60 (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Hsp60 (N-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Hsp

HSPD1

P10809

Human, Mouse, and Rat

Clone M438 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human Hsp60. This sequence has high homology to similar regions in rat and mouse Hsp60.

Hsp60 (N-terminal region)

M438

Unconjugated

ELISA, WB, ICC

Apoptosis, Cell Markers, Mitochondrial Markers

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

GOmAb

60

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:1000

This antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots of human Jurkat and rat A7r5 cells. In immunocytochemistry, anti-Hsp60 specifically stains mitochondria in paraformaldehyde fixed and NP-40 permeabilized cells.

1:100

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_3068451