Anti-Hsp70/HSPA1A/HSPA1B Antilichaam Picoband®

HSPA1 (heat shock 70kDa protein 1A)   also known as HSP70-1, HSPA1A, HSP70-1A, HSP72 or HSP70I, is a  protein  that in humans is encoded by the  HSPA1A  gene. This  intronless  gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family.  The HSPA1A gene encodes a predicted 641-amino acid protein.  The HSPA1 gene is mapped on 6p21.33. Shimizu et al. (1999)   found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of Hsp72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.

Boster Bio Anti-Hsp70/HSPA1A/HSPA1B Antibody catalog # PA1813. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Heat shock 70 kDa protein 1A ; Heat shock 70 kDa protein 1; HSP70-1 ; HSP70.1; HSPA1A; HSPA1, HSX70

HSPA1A

3303/3304

P0DMV8

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp70, different from the related mouse sequence by four amino acids and from the related rat sequence by three amino acids.

HSPA1B is testis-specific.

IHC, ICC, WB

Cancer, Chaperones, Heat Shock Proteins, Protein Trafficking, Signal Transduction, Tumor Biomarkers

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation (PubMed:23973223) . .

1. Becker, T., Hartl, F.-U., Wieland, F. CD40, an extracellular receptor for binding and uptake of Hsp70-peptide complexes. J. Cell Biol. 158: 1277-1285, 2002. 2. Gehrig, S. M., van der Poel, C., Sayer, T. A., Schertzer, J. D., Henstridge, D. C., Church, J. E., Lamon, S., Russell, A. P., Davies, K. E., Febbraio, M. A., Lynch, G. S. Hsp72 preserves muscle function and slows progression of severe muscular dystrophy. Nature 484: 394-398, 2012. 3. Shimizu, S., Nomura, K., Ujihara, M., Demura, H. An additional exon of stress-inducible heat shock protein 70 gene (HSP70-1) . Biochem. Biophys. Res. Commun. 257: 193-198, 1999.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PA1813

70052 MW

70 kDa

No cross reactivity with other proteins.

Heat shock 70 kDa protein 1A

Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Belongs to the heat shock protein 70 family.

Heat shock 70 kDa protein 1A/1B

Rabbit IgG

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.