Anti-Hu C3d APC

Complement component C3 plays a key role in the activation of complement system. In classical complement system pathway the activated C2 and C4 form classical C3-convertase (C4b2b) which cleaves C3 into C3a and C3b. In alternative complement system pathway C3 is cleaved by alternative C3-convertase (C3bBb), composed of C3b (which can be generated also by spontaneous hydrolysis of C3) and the activated form of factor B. C3b activates downstream cascade leading to formation of pores in the plasma membrane of attacked cell. C3b and its proteolytically inactive form iC3b also serve as important opsonins. C3b can generate C3f, as well as the iC3b can be further cleaved into C3c, C3d and C3g. Complement system is regulated by effective inactivation of free C3b by factor H and factor I. C3b attached to the surface of its target is protected from this inactivation. Complement system is also regulated by other proteins, e.g. CD35, CD46, CD55, or CD59. Undesired activation of complement cascade can lead to severe diseases such as PNH, rheumatoid arthritis, macular degeneration and other. Recently it has been demonstrated to take part in complications associated with Covid-19.

Human

Intact human erythrocytes coated with C3/C4

C3d

BGRL11

FC (QC tested)

APC

Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

*Dobbie D, Brazier DM, Gardner B, Holburn AM: Epitope specificities and quantitative and serologic aspects of monoclonal complement (C3c and C3d) antibodies. Transfusion 1987 Nov-Dec;27 (6) :453-9. (AGG), URL: https://pubmed.ncbi.nlm.nih.gov/2446405/, *Gankema AA, Furumaya C, Fernández-Hermira S, Hoogenboezem M, Matlung HL, van Bruggen R, Kuijpers TW: Efficient complement-mediated clearance of immunosuppressed T cells by macrophages. Front Immunol. 2023 May 16:14:1183180. (FC), URL: https://pubmed.ncbi.nlm.nih.gov/37261342/

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody BGRL11 recognizes human complement fragment C3d.

6

Mouse IgM