Anti-Hu CD158f Alexa Fluor® 488

CD158f, also known as KIR2DL5, is a polymorphic 60 kDa transmembrane glycoprotein with two Ig-like extracellular domains by which it recognize HLA class I molecules. Its long intracellular domain contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that upon extracellular ligand-mediated phosphorylation serve as docking sites for inhibitory phosphatases, which results in blocking natural cytotoxicity as well as antibody-dependent cytotoxicity of the particular NK cell, and its adhesion toward target cells. Together with other killer inhibitory receptors CD158f is important for immunological tolerance to discriminate between normal and abnormal cells. Besides NK cells it is expressed on a small population of cytotoxic T cells. Expression of CD158f alleles is highly variable in the population.

Human

Human CD158f-Ig fusion protein

CD158f

UP-R1

FC (QC tested)

Alexa Fluor® 488

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Estefanía E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J Immunol. 2007 Apr 1;178 (7) :4402-10., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=estefania+e+j+immunol+178%3A4402

*Yusa S, Catina TL, Campbell KS: KIR2DL5 can inhibit human NK cell activation via recruitment of Src homology region 2-containing protein tyrosine phosphatase-2 (SHP-2) . J Immunol. 2004 Jun 15;172 (12) :7385-92., URL: http://www.ncbi.nlm.nih.gov/pubmed/15187115, , *Du Z, Sharma SK, Spellman S, Reed EF, Rajalingam R: KIR2DL5 alleles mark certain combination of activating KIR genes. Genes Immun. 2008 Jul;9 (5) :470-80., URL: http://www.ncbi.nlm.nih.gov/pubmed/18509341

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody UP-R1 recognizes an extracellular epitope on CD158f (KIR2DL5), a 60 kDa glycoprotein serving as a HLA class I ligand, and mainly expressed on a subset of NK cells and a small population of T cells. Its expression is highly polymorphic between individuals.

6

Mouse IgG1 kappa