Anti-Hu CD195 Alexa Fluor® 488

CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.

Human

CCR5 peptide (Met1-Lys22) KLH conjugate

CD195

T21/8

ICC, FC (QC tested)

Alexa Fluor® 488

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Pollok-Kopp B, Schwarze K, Baradari VK, Oppermann M: Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278 (4) :2190-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/12403770, *Hüttenrauch F, Pollok-Kopp B, Oppermann M: G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers. J Biol Chem. 2005 Nov 11;280 (45) :37503-15., URL: http://www.ncbi.nlm.nih.gov/pubmed/16144840 , *Monde K, Maeda Y, Tanaka Y, Harada S, Yusa K: Gp120 V3-dependent impairment of R5 HIV-1 infectivity due to virion-incorporated CCR5. J Biol Chem. 2007 Dec 21;282 (51) :36923-32., URL: http://www.ncbi.nlm.nih.gov/pubmed/17971448 , *Mascalchi P, Lamort AS, Salomé L, Dumas F: Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes. Biochem Biophys Res Commun. 2012 Jan 6;417 (1) :409-13, URL: http://www.ncbi.nlm.nih.gov/pubmed?term=mascalchi%20p%20lamort%20as

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody T21/8 recognizes an extracellular epitope on the N-teminus of CD195, an approximately 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.

6

Mouse IgG1 kappa