Anti-Hu CD49a Biotine

CD49a is the alpha 1 chain of VLA integrin complex (together with CD29, serving as the beta 1 chain), and is expressed on activated T cells, monocytes, NK cells, cultured neuronal cells, melanoma cells, mesenchymal cells (including smooth muscle cells), fibroblasts, hepatocytes, and microvascular endothelium. It binds to collagen IV and laminin 1. It is important for leukocyte migration into tissues. It is upregulated in inflammatory tissues, such as inflammed intestine.

Human, Non-human primates

Human CTL line

CD49a

TS2/7

FC (QC tested), IP, IHC-F

1 mg/mL

Biotin

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Melssen MM, Olson W, Wages NA, Capaldo BJ, Mauldin IS, Mahmutovic A, Hutchison C, Melief CJ, Bullock TN, Engelhard VH, Slingluff CL: Formation and phenotypic characterization of CD49a, CD49b and CD103 expressing CD8 T cell populations in human metastatic melanoma. Oncoimmunology. 2018 Aug 6;7 (10) :e1490855., URL: https://pubmed.ncbi.nlm.nih.gov/30288359/?from_term=melssen+&from_pos=5, *Luque A, Gómez M, Puzon W, Takada Y, Sánchez-Madrid F, Cabañas C: Activated conformations of very late activation integrins detected by a group of antibodies (HUTS) specific for a novel regulatory region (355-425) of the common beta 1 chain. J Biol Chem. 1996 May 10;271 (19) :11067-75., URL: https://pubmed.ncbi.nlm.nih.gov/8626649/?from_term=luque+takada&from_pos=2, *Canfield SM, Khakoo AY: The nonintegrin laminin binding protein (p67 LBP) is expressed on a subset of activated human T lymphocytes and, together with the integrin very late activation antigen-6, mediates avid cellular adherence to laminin. J Immunol. 1999 Sep 15;163 (6) :3430-40., URL: https://pubmed.ncbi.nlm.nih.gov/10477615/?from_term=the+nonintegrin+laminin+binding&from_pos=7, *Martrus G, Kautz T, Lunemann S, Richert L, Glau L, Salzberger W, Goebels H, Langeneckert A, Hess L, Poch T, Schramm C, Oldhafer KJ, Koch M, Tolosa E, Nashan B, Altfeld M: Proliferative capacity exhibited by human liver-resident CD49a+CD25+ NK cells. PLoS One. 2017 Aug 9;12 (8) :e0182532., URL: https://pubmed.ncbi.nlm.nih.gov/28792982/?from_term=martrus+kautz&from_pos=1, *Thomson-Luque R, Wang C, Tumgia FB, Xu S, Szekeres K, Conway A, Adapa SR, Barnes SJ, Adams JH, Jiang RH: In-depth phenotypic characterization of reticulocyte maturation using mass cytometry. Blood Cells Mol Dis. 2018 Sep;72:22-33., URL: https://pubmed.ncbi.nlm.nih.gov/30007855/?from_term=in-depth+phenotypic+characterization+of+reticulocyte&from_pos=1, *Marquardt N, Kekäläinen E, Chen P, Lourda M, Wilson JN, Scharenberg M, Bergman P, Al-Ameri M, Hård J, Mold JE, Ljunggren HG, Michaëlsson J. Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells. Nat Commun. 2019 Aug 26;10 (1) :3841., URL: https://pubmed.ncbi.nlm.nih.gov/31451696/?from_term=marquardt+n+chen+p&from_pos=1, *Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20 (8) :1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/, *Hemler ME, Jacobson JG, Strominger JL: Biochemical characterization of VLA-1 and VLA-2. Cell surface heterodimers on activated T cells. J Biol Chem. 1985 Dec 5;260 (28) :15246-52., URL: https://pubmed.ncbi.nlm.nih.gov/2415516/

Store at 2-8°C. Do not freeze.

The mouse monoclonal antibody TS2/7 recognizes an extracellular epitope of CD49a, the alpha 1 chain of VLA integrin complex, that serves as an adhesion receptor for collagen IV and laminin 1.

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Mouse IgG1