Anti-Hu IgE HRP

Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.

Human

Purified human IgE.

IgE

BE5

ELISA (QC tested)

0.2 mg/mL

HRP

Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal

*Kaulfürst-Soboll H, Mertens M, Brehler R, von Schaewen A: Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis. PLoS One. 2011 Mar 14;6 (3) :e17800., URL: http://www.ncbi.nlm.nih.gov/pubmed/21423762, *Maerle AV, Simonova MA, Pivovarov VD, Voronina DV, Drobyazina PE, Trofimov DY, Alekseev LP, Zavriev SK, Ryazantsev DY: Development of the covalent antibody-DNA conjugates technology for detection of IgE and IgM antibodies by immuno-PCR. PLoS One 2019 Jan 4;14 (1) :e0209860., URL: https://pubmed.ncbi.nlm.nih.gov/30608947/

*Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh) . 1975;194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690?ordinalp, *Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21 (6) :667-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/1122610?ordina, *Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39 (2) :129-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/6966908?ordina, *Gould HJ, Beavil RL, Vercelli D: IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation. Br Med Bull. 2000;56 (4) :908-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/11359628?ordin, *Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152 (2) :323-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/8258141?ordina

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The antibody BE5 reacts with human IgE; it recognizes an epitope different from the ones recognized by 4G7.325 and 4H10 antibodies to IgE.

Mouse IgG1