Anti-IκBα (C-terminus) Antilichaam

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.

Our IκBα (C-terminus) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat IκBα (C-terminus) and is antigen affinity purified. It is great for use in ELISA, WB.

Our IκBα (C-terminus) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat IκBα (C-terminus) and is antigen affinity purified. It is great for use in WB.

IkB, MAD3, IkappaBalpha, NFkappaB inhibitor IkBa

NFKBIA

P25963

Human, Mouse, and Rat

IκBα (C-terminus) synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus of human IκBα. This peptide sequence has high homology to rat and mouse IκBα, and low homology to other IκB proteins.

IκBα (C-terminus)

Unconjugated

ELISA, WB

Cell Signaling, Oncoproteins, Transcription Regulation

Antigen Affinity Purified

PBS + 0.05% NaN3 and 50% glycerol

38

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

This antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of human A431 and Jurkat cells, as well as mouse C2C12 cells.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_2151401