Anti-βIII-Tubuline Antilichaam

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro. Unphosphorylated Ser-444 in βIII-Tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in β-Tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.

Our unphosphorylated βIII-Tubulin (Ser-444) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat unphosphorylated βIII-Tubulin (Ser-444) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Our unphosphorylated βIII-Tubulin (Ser-444) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat unphosphorylated βIII-Tubulin (Ser-444) and is antigen affinity purified. It is great for use in WB, ICC.

TUBB3

TUBB3

Q13509

Human, Mouse, and Rat

Unphosphorylated βIII-Tubulin synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 444 of human βIII-Tubulin. This sequence is not found in bI or βII-Tubulin isotypes, but is well conserved in βIII-Tubulins from rat and mouse.

Unphosphorylated βIII-Tubulin (Ser-444)

Unconjugated

ELISA, WB, ICC

Cell Adhesion Molecules, Cell Signaling, Cytoskeletal Regulation

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

50

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

This antibody was cross-adsorbed to phospho-βIII-Tubulin (Ser-444) peptide before affinity purification using unphosphorylated βIII-Tubulin (Ser-444) peptide (without carrier) . The antibody detects a 50 kDa* protein corresponding to the molecular mass of unphosphorylated βIII-Tubulin on SDS-PAGE immunoblots of purified brain tubulin and mouse brain tissue.

1:100

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_2210669