Anti-integrine beta 4 bindingseiwit/EIF6 Antibody Picoband® fluoro550 Vervoeging

EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al. (1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al. (2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al. (2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50% reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

Eukaryotic translation initiation factor 6 ; eIF-6 ; B (2) GCN homolog; B4 integrin interactor; CAB; p27 (BBP) ; EIF6 ; EIF3A, ITGB4BP ; OK/SW-cl.27

EIF6

3692

P56537

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human EIF6, different from the related rat and mouse sequences by one amino acid.

Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level) . .

Flow Cytometry

Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Immunogen affinity purified.

Liquid

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1) -dependent protein kinase C activity. .

1. Ceci, M., Gaviraghi, C., Gorrini, C., Sala, L. A., Offenhauser, N., Marchisio, P. C., Biffo, S. Release of eIF6 (p27-BBP) from the 60S subunit allows 80S ribosome assembly. Nature 426: 579-584, 2003. 2. Gandin, V., Miluzio, A., Barbieri, A. M., Beugnet, A., Kiyokawa, H., Marchisio, P. C., Biffo, S. Eukaryotic initiation factor 6 is rate-limiting in translation, growth and transformation. Nature 455: 684-688, 2008. 3. Sanvito, F., Arrigo, G., Zuffardi, O., Agnelli, M., Marchisio, P. C., Biffo, S. Localization of p27 beta-4 binding protein gene (ITGB4BP) to human chromosome region 20q11.2. Genomics 52: 111-112, 1998.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

26599 MW

No cross reactivity with other proteins.

6

Eukaryotic translation initiation factor 6

Cytoplasm. Nucleus, nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.

Belongs to the eIF-3 subunit B family.

Eukaryotic translation initiation factor 6

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.