Anti-Interleukin-10 IL10 Antibody Picoband® fluoro488 Vervoeging

Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10

IL10

16153

P18893

Mouse

No cross-reactivity with other proteins

E. coli-derived mouse IL-10 recombinant protein (Position: S19-S178) .

Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Flow Cytometry

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Immunogen affinity purified.

Liquid

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

1. Eskdale J, Kube D, Tesch H, Gallagher G (1997) . Mapping of the human IL10 gene and further characterization of the 5' flanking sequence.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

IL10 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL10 Antibody (RP1014) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

No cross reactivity with other proteins.

6

Interleukin-10

Secreted.

Belongs to the IL-10 family.

Interleukin-10 (IL-10)

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.