Anti-Internexine alfa (Alfa-Inx) Antilichaam

Neurofilaments can be defined as the intermediate or 10 nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.

Our Anti-Internexin alpha (Alpha-Inx) rabbit polyclonal primary antibody detects cat, human, mouse, other mammals, and rat Internexin alpha (Alpha-Inx), and is whole serum. It is validated for use in ICC, IHC-Frozen, WB.

Our Anti-Internexin alpha (Alpha-Inx) rabbit polyclonal primary antibody detects cat, human, mouse, other mammals, and rat Internexin alpha (Alpha-Inx), and is whole serum. It is validated for use in ICC, IHC-Frozen, WB.

67 kDa neurofilament protein; NF-66; Neurofilament-66; Alpha-internexin; Alpha-Inx; Neurofilament 5; INA; NEF5

P23565

Cat, Human, Mouse, Other Mammals, and Rat

Recombinant alpha-internexin expressed and purified from E. coli

Internexin alpha (Alpha-Inx)

Unconjugated

ICC, IHC-Frozen, WB

Whole serum

Lyophilized with sodium azide.

64-66 kDa

For research use only.

25°C (ambient)

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

1:500-1:1000

1:10000-1:20000

The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Hu, rat, Ms, Fel, and other mammals.

1:500-1:1000

Rabbit

Lyophilized

Mixed

Rat