Anti-Ki-67 Antilichaam

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226) . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226) . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226) . Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551) . Does not contribute to the internal structure of mitotic chromosomes (By similarity) . May play a role in chromatin organization (PubMed:24867636) . It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable) . Ref: uniprot.org

Our Anti-Ki-67 mouse monoclonal primary antibody detects human Ki-67 , and is affinity-purified. It is validated for use in ICC, WB.

Our Anti-Ki-67 mouse monoclonal primary antibody detects human Ki-67, and is affinity-purified. It is validated for use in ICC, WB.

KI-67

P46013

Human

E. coli.

Ki-67

6B4

Unconjugated

ICC, WB

Affinity-purified

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide.

For research use only.

25°C (ambient)

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.

Protein G purified

1:2000-1:5000

1:1000-1:5000

Reacts with human only. Does not react with mouse or rat.

Mouse

Lyophilized

IgG1

Human