Anti-Krueppel-achtige factor 8 KLF8 Antibody Picoband® fluoro647 Vervoeging

KLF8 (Krueppel-like factor 8) is a protein that in humans is encoded by the KLF8 gene. It is also named as ZNF741, or BKLF3. Kruppel-like factor proteins, including KLF8, all contain a characteristic C-terminal DNA-binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. The International Radiation Hybrid Mapping Consortium mapped the KLF8 gene to the X chromosome (WI-18614) . Suske et al. (2005) stated that the human KLF8 gene maps to chromosome Xp11.21, and the mouse Klf8 gene to chromosome XF3. van Vliet et al. (2000) identified a cDNA encoding KLF8, also called ZNF741. Expression of KLF8 or only its zinc fingers showed that the fingers could bind to a CACCC box. Sequence analysis predicted that the 359-amino acid protein contains a nuclear localization signal upstream of the zinc fingers, a valine-rich hydrophobic stretch, and a pro-val-asp-leu-ser (PVDLS) motif. Yeast 2-hybrid and GST pull-down analysis demonstrated that CTBP2 interacts with KLF8 through the PVDLS motif.

Krueppel-like factor 8; Basic krueppel-like factor 3; Zinc finger protein 741; KLF8; BKLF3, ZNF741

KLF8

11279

O95600

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the N-terminus of human KLF8.

Ubiquitous. .

Flow Cytometry

Domain Families, Epigenetics and Nuclear Signaling, Krueppel Like Factor, Transcription, Zinc Finger

Immunogen affinity purified.

Liquid

Transcriptional repressor and activator. Binds to CACCC- boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G (1) phase as a downstream target of focal adhesion kinase (FAK) . .

1. Lossi, A.-M., Laugier-Anfossi, F., Depetris, D., Gecz, J., Gedeon, A., Kooy, F., Schwartz, C., Mattei, M.-G., Croquette, M.-F., Villard, L.Abnormal expression of the KLF8 (ZNF741) gene in a female patient with an X; autosome translocation t (X;21) (p11.2; q22.3) and non-syndromic mental retardation.J. Med. Genet. 39: 113-117, 2002. 2. Suske, G., Bruford, E., Philipsen, S.Mammalian SP/KLF transcription factors: bring in the family.Genomics 85: 551-556, 2005. 3. van Vliet, J., Turner, J., Crossley, M.Human Kruppel-like factor 8: a CACCC-box binding protein that associates with CtBP and represses transcription.Nucleic Acids Res. 28: 1955-1962, 2000.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

39314 MW

No cross reactivity with other proteins.

6

Krueppel-like factor 8

Nucleus .

Belongs to the Sp1 C2H2-type zinc-finger protein family.

Krueppel-like factor 8

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.