Anti-Lamine B1/LMNB1 Antibody Picoband®

Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Boster Bio Anti-Lamin B1/LMNB1 Antibody catalog # PA1048. Tested in IP, ICC/IF, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Lamin-B1; LMNB1; LMN2, LMNB

LMNB1

4001

P20700

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1, different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids.

Widely expressed in non-excitable tissues.

IF, ICC/IF, IHC, WB

Apoptosis, Cancer, Cell Biology, Cell Death, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Nucleus, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

1. Pollard, K. M.; Chan, E. K.; Grant, B. J.; Sullivan, K. E.; Tan, E. M.; Glass, C. A. : In vitro posttranslational modification of lamin B clones from a human T-cell line. Molec. Cell. Biol. 10: 2164-2175, 1990 2. Tsai, M.-Y.; Wang, S.; Heidinger, J. M.; Shumaker, D. K.; Adam, S. A.; Goldman, R. D.; Zheng, Y. : A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science 311: 1887-1893, 2006.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PA1048

66 kDa

70-72 kDa

No cross reactivity with other proteins.

Lamin-B1

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.

Belongs to the intermediate filament family.

Lamin-B1

Rabbit IgG

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.