Anti-LIN28 LIN28A Monoklonale Antilichaam

Boster Bio Anti-LIN28 LIN28A Monoclonal Antibody catalog # M01966-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Protein lin-28 homolog A; Lin-28A; Zinc finger CCHC domain-containing protein 1; LIN28A; CSDD1, LIN28, ZCCHC1

LIN28A

79727

Q9H9Z2

Human

A synthesized peptide derived from human LIN28 Involved in the timing of developmental events and choice of stage specific cell fates. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7.

Clone: AFFE-12

Expressed in embryonic stem cells, placenta and testis. Tends to be up-regulated in HER2-overexpressing breast tumors. .

WB, IHC, ICC, IF, IP, Flow Cytometry

DNA/RNA, Epigenetics and Nuclear Signaling, Translation

Affinity-chromatography

0.5mg/ml

Liquid

'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, inly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity) . Suppressor of microRNA (miRNA) biogenesis, including that of let- 7, miR107, miR-143 and miR-200c. Specifically binds miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits ZCCHC11/TUT4 uridylyltransferase. This results in the terminal uridylation of target pre-miRNAs (PubMed:19703396) (PubMed:22118463) (PubMed:22898984) . Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (By similarity) . .

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=M01966-1

22743 MW

33 kDa

Protein lin-28 homolog A

Cytoplasm . Nucleus, nucleolus. Predominantly cytoplasmic (PubMed:22118463) . Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity) . Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules. .

Lysosome-associated membrane glycoprotein 2 (Lamp2)

Rabbit IgG

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.