Anti-MAGED4 Antibody Picoband® 0,00647 Vervoeging

MAGE family member D4 is a protein that in humans is encoded by the MAGED4 gene. MAGED4, originally termedMAGEE1, is a member ofMAGE (Melanoma associated antigen) super-family identified by serial analysis of gene expression (SAGE) technique (PMID: 11406556; 24966945) . Three isoforms (MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription (PMID: 11406556) . This antibody raised against 1-350 aa of humanMAGED4 protein detects a band of 90-100 kDa, which islargerthan the calculated molecularweight. The slower electrophoretic mobility may be due to abnormal spatial conformation ofMAGED4 protein caused by abundance of proline in composition (PMID: 24966945) .

Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

MAGED4

728239

Q96JG8

Human

No cross-reactivity with other proteins.

E.coli-derived human MAGED4 recombinant protein (Position: F51-R741) .

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Flow Cytometry

Cell Biology

Immunogen affinity purified.

Liquid

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

1. Chomez, P., De Backer, O., Bertrand, M., De Plaen, E., Boon, T., Lucas, S. An overview of the MAGE gene family with the identification of all human members of the family. Cancer Res. 61: 5544-5551, 2001. [PubMed: 11454705, related citations] 2. Hartz, P. A. Personal Communication. Baltimore, Md. 4/30/2009. 3. Ito, S., Kawano, Y., Katakura, H., Takenaka, K., Adachi, M., Sasaki, M., Shimizu, K., Ikenaka, K., Wada, H., Tanaka, F. Expression of MAGE-D4, a novel MAGE family antigen, is correlated with tumor-cell proliferation of non-small cell lung cancer. Lung Cancer 51: 79-88, 2006.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

24145 MW

6

Kelch repeat and BTB domain containing 2

Cul3-RING ubiquitin ligase complex.

Transmembrane protein 240

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.