Anti-Mast Cell Chymase/CMA1 Antibody Picoband®

Chymase is a major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. Chymase is mapped to 14q12. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II.

Boster Bio Anti-Mast Cell Chymase/CMA1 Antibody Picoband® catalog # PB9055. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Chymase;3.4.21.39; Alpha-chymase; Mast cell protease I; CMA1; CYH, CYM

CMA1

1215

P23946

Human, Mouse, Rat

No cross-reactivity with other proteins

E.coli-derived human CMA1 recombinant protein (Position: I22-N247) . Human CMA1 shares 75% and 74% amino acid (aa) sequences identity with mouse and rat CMA1, respectively.

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. .

IHC, WB

Atherosclerosis, Cardiovascular, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Immunology, Innate Immunity, Macrophage/Inflammation, Signal Transduction, Vascular Inflammation

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

1. Caughey, GH. Mast cell tryptases and chymases in inflammation and host defense. Immu Revs 2007 (217) : 141-154. 2. Chmelar, J., Oliveira, C. J., Rezacova, P., Francischetti, I. M. B., Kovarova, Z., Pejler, G., Kopacek, P., Ribeiro, J. M. C., Mares, M., Kopecky, J., Kotsyfakis, M. A tick salivary protein targets cathepsin G and Chymase and inhibits host inflammation and platelet aggregation. Blood 117: 736-744, 2011. 3. Urata, H., Boehm, K. D., Philip, A., Kinoshita, A., Gabrovsek, J., Bumpus, F. M., Husain, A.Cellular localization and regional distribution of an angiotensin II-forming Chymase in the heart. J. Clin. Invest. 91: 1269-1281, 1993.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PB9055

27325 MW

35 kDa

No cross reactivity with other proteins.

Chymase

Secreted. Cytoplasmic granule. Mast cell granules.

Belongs to the peptidase S1 family. Granzyme subfamily.

Chymase

Rabbit IgG

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.