Anti-MCM3 Antibody Picoband® fluoro488 Vervoeging

MCM3 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 3), also called RLFB or P1 PROTEIN, is a protein that in humans is encoded by the MCM3 gene. MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The MCM3 gene is mapped to 6p12.2. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact ly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

DNA replication licensing factor MCM3;3.6.4.12; DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102; MCM3

MCM3

4172

P25205

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3, identical to the related rat and mouse sequences.

Heart, liver, duodenum, blood vessels and kidney.

Flow Cytometry

DNA Synthesis, Epigenetics and Nuclear Signaling

Immunogen affinity purified.

Liquid

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 2. Thommes P, Fett R, Schray B, Burkhart R, Barnes M, Kennedy C, Brown NC, Knippers R (Apr 1992) . Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein. Nucleic Acids Res 20 (5) : 1069–74. 3. Entrez Gene: MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) .

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

90981 MW

No cross reactivity with other proteins.

6

DNA replication licensing factor MCM3

Nucleus.

Belongs to the MCM family.

DNA replication licensing factor MCM3

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.