Anti-MCM5 Antibody Picoband® 31.0550 Vervoeging

MCM5 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 5), also known as CDC46, is a protein that in humans is encoded by the MCM5 gene. MCM5 is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. And MCM5 is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The MCM5 gene is mapped on 22q12.3. The MCM5 gene contains 16 exons. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

DNA replication licensing factor MCM5;3.6.4.12; CDC46 homolog; P1-CDC46; MCM5; CDC46

MCM5

4174

P33992

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human MCM5, identical to the related rat and mouse sequences.

Heart, liver, duodenum, blood vessels and kidney.

Flow Cytometry

DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Immunogen affinity purified.

Liquid

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity) . Interacts with MCMBP. .

1. Burkhart, R., Schulte, D., Hu, D., Musahl, C., Gohring, F., Knippers, R. Interactions of human nuclear proteins P1Mcm3 and P1Cdc46. Europ. J. Biochem. 228: 431-438, 1995. 2. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 3. Paul R, Hu B, Musahl C, Hameister H, Knippers R (Sep 1996) . Coding sequence and chromosome mapping of the human gene (CDC46) for replication protein hCdc46/Mcm5. Cytogenet Cell Genet 73 (4) : 317–21.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

82286 MW

No cross reactivity with other proteins.

6

DNA replication licensing factor MCM5

Nucleus.

Belongs to the MCM family.

DNA replication licensing factor MCM5

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.