Anti-MED7 Antibody Picoband® 0,00647 Vervoeging

Mediator of RNA polymerase II transcription subunit 7 is an enzyme that in humans is encoded by the MED7 gene. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.

MED7

9443

O43513

Human, Mouse, Rat

E.coli-derived human MED7 recombinant protein (Position: M1-P233) . Human MED7 shares 97% amino acid (aa) sequence identity with mouse MED7.

Flow Cytometry

Immunogen affinity purified.

Liquid

1. Myers, L. C., Gustafsson, c. M., Bushnell, D. A., Lui, M., Erdjument-Bromage, H., Tempst, P., Kornberg, R. D. The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev. 12: 45-54, 1998. 2. Ryu, S., Zhou, S., Ladurner, A. G., Tjian, R. The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1. Nature 397: 446-450, 1999. 3. Sato, S., Tomomori-Sato, C., Parmely, T. J., Florens, L., Zybailov, B., Swanson, S. K., Banks, C. A. S., Jin, J., Cai, Y., Washburn, M. P., Conaway, J. W., Conaway, R. C. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Molec. Cell 14: 685-691, 2004.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

27 kDa

6

IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.