Anti-MEK4/MAP2K4 Antibody Picoband® fluoro488 Vervoeging

Dual specificity mitogen-activated protein kinase kinase 4 (MAP2K4), also called SEK1 or JNKK1, is an enzyme that in humans is encoded by the MAP2K4 gene. It is mapped to 17p12. This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK1. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. MAP2K4 was a specific activator of JNK1, JNK2, and p38, and it has been shown to interact with FLNC, MAPK8, MAPK8IP3 and AKT1.

Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4;2.7.12.2; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK; MAP2K4; JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1

MAP2K4

6416

P45985

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human MEK4, different from the related mouse and rat sequences by one amino acid.

Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

Flow Cytometry

MAPK Pathway, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Immunogen affinity purified.

Liquid

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to ly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. .

1. Entrez Gene: MAP2K4 mitogen-activated protein kinase kinase 4 2. Lin A, Minden A, Martinetto H, Claret FX, Lange-Carter C, Mercurio F, Johnson GL, Karin M (May 1995) . Identification of a dual specificity kinase that activates the Jun kinases and p38-Mpk2.Science 268 (5208) : 286–90.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

44288 MW

No cross reactivity with other proteins.

6

Dual specificity mitogen-activated protein kinase kinase 4

Cytoplasm . Nucleus .

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Dual specificity mitogen-activated protein kinase kinase 4

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.