Anti-MEKK1/MAP3K1 Antibody Picoband® fluoro488 Vervoeging

MAP3K1 (Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al. (1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al. (1998) showed that human MEKK1 activated JNK1 (MAPK8) robustly and p38-alpha (MAPK14) less efficiently, but it had only a marginal effect on ERK2 (MAPK1) . MEKK1 ly and specifically interacted with JNKK1 (MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al. (1998) concluded that JNKK1 is the preferred MEKK1 substrate.

Mitogen-activated protein kinase kinase kinase 1;2.7.11.25; MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1; MAP3K1; MAPKKK1, MEKK, MEKK1

MAP3K1

4214

Q13233

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK1, identical to the related rat and mouse sequences.

Flow Cytometry

MAPK Pathway, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Immunogen affinity purified.

Liquid

Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. .

1. Juriloff, D. M., Harris, M. J., Mah, D. G. The open-eyelid mutation, lidgap-Gates, is an eight-exon deletion in the mouse Map3k1 gene. Genomics 85: 139-142, 2005. 2. Vinik, B. S., Kay, E. S., Fiedorek, F. T., Jr. Mapping of the MEK kinase gene (Mekk) to mouse chromosome 13 and human chromosome 5. Mammalian Genome 6: 782-783, 1995. 3. Xia, Y., Wu, Z., Su, B., Murray, B., Karin, M. JNKK1 organizes a MAP kinase module through specific and sequential interactions with upstream and downstream components mediated by its amino-terminal extension. Genes Dev. 12: 3369-3381, 1998.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

164 kDa

No cross reactivity with other proteins.

6

Mitogen-activated protein kinase kinase kinase 1

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Mitogen-activated protein kinase kinase kinase 1

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.