Anti-MEKK3/MAP3K3 Antibody Picoband® fluoro594 Vervoeging

MAP3K3 (Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17 (Puffenberger et al., 2007) . By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al. (2003) showed that a C-terminal fragment of mouse Osm (CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted ly with the Mekk3 substrate Mkk3 (MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al. (2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.

Mitogen-activated protein kinase kinase kinase 3;2.7.11.25; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3; Map3k3; Mekk3

MAP3K3

26406

Q61084

Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the N-terminus of mouse MEKK3, different from the related rat sequence by two amino acids.

Flow Cytometry

Immunology, Innate Immunity, MAPK Pathway, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, TLR Signaling

Immunogen affinity purified.

Liquid

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. .

1. Channavajhala, P. L., Rao, V. R., Spaulding, V., Lin, L. L., Zhang, Y. G. hKSR-2 inhibits MEKK3-activated MAP kinase and NF-kappa-B pathways in inflammation. Biochem. Biophys. Res. Commun. 334: 1214-1218, 2005. 2. Puffenberger, E. G., Strauss, K. A., Ramsey, K. E., Craig, D. W., Stephan, D. A., Robinson, D. L., Hendrickson, C. L., Gottlieb, S., Ramsay, D. A., Siu, V. M., Heuer, G. G., Crino, P. B., Morton, D. H. Polyhydramnios, megalencephaly and symptomatic epilepsy caused by a homozygous 7-kilobase deletion in LYK5. Brain 130: 1929-1941, 2007. 3. Uhlik, M. T., Abell, A. N., Johnson, N. L., Sun, W., Cuevas, B. D., Lobel-Rice, K. E., Horne, E. A., Dell'Acqua, M. L., Johnson, G. L. Rac-MEKK3-MKK3 scaffolding for p38 MAPK activation during hyperosmotic shock. Nature Cell Biol. 5: 1104-1110, 2003.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

70776 MW

No cross reactivity with other proteins.

6

Mitogen-activated protein kinase kinase kinase 3

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Mitogen-activated protein kinase kinase kinase 3

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.