Anti-MGAT3 Antilichaam

Boster Bio Anti-MGAT3 Antibody catalog # A08470. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase ;2.4.1.144

MGAT3

Q09327

Human, Mouse, Rat

Recombinant fusion protein of human MGAT3 (NP_002400.3) .

WB

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

1 mg/mL

Liquid

It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains, called bisecting N-acetylglucosamine (GlcNAc) . It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. The addition of this bisecting GlcNAc residue alters not only the composition, but also the conformation of the N-glycan. The introduction of the bisecting GlcNAc residue results in the suppression of further processing and elongation of N-glycans, precluding the formation of beta-1,6 GlcNAc branching, catalyzed by MGAT5 since it is unable to use the bisected oligosaccharide as a substrate (PubMed:19403558) . Addition of bisecting N-acetylglucosamine to CDH1/E-cadherin modulates CDH1 cell membrane location (PubMed:19403558) . Inhibits NeuAc-alpha-2,3-Gal-beta-1,4- GlcNAc- formation which modulates sialylation levels and plays a role in cell migration regulation (PubMed:26801611) . In brain, addition of bisecting N-acetylglucosamine to BACE1 blocks its lysosomal targeting in response to oxidative stress and further degradation which increases its location to early endosome and the APP cleavage (By similarity) .

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=A08470

61313 MW

WB: 1:500-1:2000

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Golgi apparatus membrane; Single-pass type II membrane protein .

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2