Anti-Milk Fat Globule 1/MFGE8 Antibody Picoband®

MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al. (1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al. (2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al. (2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE) -dependent mechanism.

Boster Bio Anti-Milk Fat Globule 1/MFGE8 Antibody catalog # PA1945. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Lactadherin; Breast epithelial antigen BA46; HMFG; MFGM; Milk fat globule-EGF factor 8; MFG-E8; SED1; Lactadherin short form; Medin; MFGE8

MFGE8

4240

Q08431

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human Human Milk Fat Globule 1.

Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.

IHC, WB

Angiogenesis, Apoptosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Metabolism, Metabolism Processes, Pathways and Processes

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity) . Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. .

1. Bu, H.-F., Zuo, X.-L., Wang, X., Ensslin, M. A., Koti, V., Hsueh, W., Raymond, A. S., Shur, B. D., Tan, X.-D. Milk fat globule-EGF factor 8/lactadherin plays a crucial role in maintenance and repair of murine intestinal epithelium. J. Clin. Invest. 117: 3673-3683, 2007. 2. Collins, C., Nehlin, J. O., Stubbs, J. D., Kowbel, D., Kuo, W.-L., Parry, G. Mapping of a newly discovered human gene homologous to the apoptosis associated-murine mammary protein, MFG-E8, to chromosome 15q25. Genomics 39: 117-118, 1997. 3. Hanayama, R., Tanaka, M., Miwa, K., Shinohara, A., Iwamatsu, A., Nagata, S. Identification of a factor that links apoptotic cells to phagocytes. Nature 417: 182-187, 2002.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PA1945

43123 MW

43 kDa

No cross reactivity with other proteins.

Lactadherin

Membrane ; Peripheral membrane protein . Secreted .

Contains 1 EGF-like domain.

Lactadherin

Rabbit IgG

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.