Anti-MPG Antibody Picoband® fluoro488 Vervoeging

MPG (N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines.  The  MPG  gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29) . The completely characterized human  MPG  gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo,   Hang et al. (1997)   biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.

DNA-3-methyladenine glycosylase;3.2.2.21;3-alkyladenine DNA glycosylase;3-methyladenine DNA glycosidase; ADPG; N-methylpurine-DNA glycosylase; MPG; AAG, ANPG, MID1

MPG

4350

P29372

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of human MPG, different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid.

Expressed in breast, ovary and prostate.

Flow Cytometry

DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling

Immunogen affinity purified.

Liquid

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

1. Boosalis, M., Derfler, B., Call, K., Samson, L. Cloning of a human 3-methyladenine DNA glycosylase cDNA whose gene maps to human chromosome 16. (Abstract) Am. J. Hum. Genet. 49 (suppl.) : 399 only, 1991. 2. Engelward, B. P., Weeda, G., Wyatt, M. D., Broekhof, J. L. M., de Wit, J., Donker, I., Allan, J. M., Gold, B., Hoeijmakers, J. H. J., Samson, L. D.Base excision repair deficient mice lacking the Aag alkyladenine DNA glycosylase. Proc. Nat. Acad. Sci. 94: 13087-13092, 1997. 3. Kielman, M. F., Smits, R., Bernini, L. F. Localization and characterization of the mouse alpha-globin locus control region. Genomics 21: 431-433, 1994.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

32869 MW

No cross reactivity with other proteins.

6

DNA-3-methyladenine glycosylase

Cytoplasm . Mitochondrion matrix, mitochondrion nucleoid . Nucleus .

Belongs to the DNA glycosylase MPG family.

DNA-3-methyladenine glycosylase

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.