Anti-MPXV A29L Human IgG ELISA Kit

PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant MPXV A29L antigen has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-MPXV A29L Human IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-MPXV A29L Human IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Used for the quantitative determination of Anti-MPXV A29L Human IgG concentration in serum and plasma.

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 °C, the rest reagents should be store at 4°C.

2-8 °C

For Research Use Only.