Anti-MRPS25 Antibody Picoband®

28S ribosomal protein S25, mitochondrial is a protein that in humans is encoded by the MRPS25 gene. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants.

Boster Bio Anti-MRPS25 Antibody Picoband® catalog # A14080-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

RNA-binding protein 47; RNA-binding motif protein 47; RBM47

MRPS25

64432

P82663

Human, Mouse, Rat

No cross-reactivity with other proteins

E.coli-derived human MRPS25 recombinant protein (Position: M1-D173) .

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Chromatin Binding Proteins, DNA/RNA Binding, Epigenetics and Nuclear Signaling

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

1. Bugiardini, E., Mitchell, A. L., Dalla Rosa, I., Horning-Do, H.-T., Pitmann, A. M., Poole, O. V., Holton, J. L., Shah, S., Woodward, C., Hargreaves, I., Quinlivan, R., Amunts, A., Wiesner, R. J., Houlden, H., Holt, I. J., Hanna, M. G., Pitceathly, R. D. S., Spinazzola, A. MRPS25 mutations impair mitochondrial translation and cause encephalomyopathy. Hum. Molec. Genet. 28: 2711-2719, 2019. 2. Kenmochi, N., Suzuki, T., Uechi, T., Magoori, M., Kuniba, M., Higa, S., Watanabe, K., Tanaka, T. The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders. Genomics 77: 65-70, 2001. 3. Koc, E. C., Burkhart, W., Blackburn, K., Moseley, A., Spremulli, L. L. The small subunit of the mammalian mitochondrial ribosome: identification of the full complement of ribosomal proteins present. J. Biol. Chem. 276: 19363-19374, 2001.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

https://www.bosterbio.com/msds?sku=A14080-3

64099 MW

20 kDa

6

RNA-binding protein 47

Nucleus .

Follicular dendritic cell secreted peptide

Rabbit IgG

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.