Anti-MSH6/Gtbp Konijn Monoklonale Antilichaam

Boster Bio Anti-MSH6/Gtbp Rabbit Monoclonal Antibody catalog # M00553. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

DNA mismatch repair protein Msh6 ; hMSH6 ; G/T mismatch-binding protein ; GTBP ; GTMBP ; MutS protein homolog 6 ; MutS-alpha 160 kDa subunit; p160 ; MSH6 ; GTBP

MSH6

2956

P52701

Human, Mouse, Rat

A synthesized peptide derived from human MSH6

Clone: AEB-13

Ubiquitous.

WB, IHC, ICC, IF

DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling

Affinity-chromatography

0.5mg/ml

Liquid

Component of the post-replicative DNA mismatch repair system (MMR) . Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for ing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3) : early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction. .

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=M00553

152786 MW

42 kDa, 48 kDa

DNA mismatch repair protein Msh6

Nucleus . Chromosome . Associates with H3K36me3 via its PWWP domain.

T-cell surface glycoprotein CD4

Rabbit IgG

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.