Anti-MTHFD1 Antibody Picoband® fluoro550 Vervoeging

Monofunctional C1-tetrahydrofolate synthase, mitochondrial also known as formyltetrahydrofolate synthetase, is an enzyme that in humans is encoded by the MTHFD1L gene (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like) . The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.

Tripartite motif-containing protein 6; RING finger protein 89; RING-type E3 ubiquitin transferase TRIM6; TRIM6; RNF89

MTHFD1

4522

P11586

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human MTHFD1 recombinant protein (Position: K21-Q855) .

Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. .

Flow Cytometry

Signal Transduction

Immunogen affinity purified.

Liquid

E3 ubiquitin-protein ligase which ubiquitinates MYC and inhibits its transcription activation activity, maintaining the pluripotency of embryonic stem cells. Involved in the synthesis of unanchored K48-linked polyubiquitin chains which interact with and activate the serine/threonine kinase IKBKE, leading to phosphorylation of STAT1 and stimulation of an antiviral response .

1. Christensen, K. E., Patel, H., Kuzmanov, U., Mejia, N. R., MacKenzie, R. E. Disruption of the Mthfd1 gene reveals a monofunctional 10-formyltetrahydrofolate synthetase in mammalian mitochondria. J. Biol. Chem. 280: 7597-7602, 2005. 2. Momb, J., Lewandowski, J. P., Bryant, J. D., Fitch, R., Surman, D. R., Vokes, S. A., Appling, D. R. Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice. Proc. Nat. Acad. Sci. 110: 549-554, 2013. 3. Prasannan, P., Pike, S., Peng, K., Shane, B., Appling, D. R. Human mitochondrial C (1) -tetrahydrofolate synthase: gene structure, tissue distribution of the mRNA, and immunolocalization in Chinese hamster ovary cells. J. Biol. Chem. 278: 43178-43187, 2003.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

37492 MW

6

Tripartite motif containing 6

Cytoplasm.

Tripartite motif-containing protein 6

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.