Anti-MuRF1 (C-terminaal gebied) Antilichaam

Muscle proteolysis is regulated by the ATP-dependent ubiquitin–proteasome system. This system involves ubiquitination of specific proteins, leading to recognition and degradation by the 26S proteasome complex. Ubiquitination requires interactions with ubiquitin related proteins, ubiquitin-activating (E1), ubiquitin-conjugating (E2) and ubiquitin-ligating enzymes (E3) known as ligases. Two muscle specific ubiquitin ligases have been identified, muscle ring finger 1 (MuRF-1) and Atrogin 1. Both ligases are regulated by the Akt1/FOXO1 signaling pathway, and both proteins have been shown to be upregulated prior to the onset of atrophy in multiple models of muscle wasting, including disuse and cachexia. MuRF1 is also known as TRIM63, SMRZ, and RNF28, and its expression is upregulated after TNFα treatment in C2C12 cells and muscle tissue, while localization of MuRF1 protein has been observed in the cytoplasm and nucleus of cells.

Our MuRF1 (C-terminal region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat MuRF1 (C-terminal region) and is antigen affinity purified. It is great for use in ELISA, WB.

Our MuRF1 (C-terminal region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat MuRF1 (C-terminal region) and is antigen affinity purified. It is great for use in WB.

Tripartite motif 63, TRIM63, SMRZ, IRF, RNF28, Muscle RING finger, MURF-1

TRIM63

Q969Q1

Human, Mouse, and Rat

Clone M316 was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1. This peptide sequence is highly conserved in rat and mouse MuRF1, and has 50% homology to MuRF2 (TRIM-55) .

MuRF1 (C-terminal region)

M316

Unconjugated

ELISA, WB

Neurodegenerative Disease, Parkinson's Disease, Amyotrophic Lateral Sclerosis (ALS)

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

30/38

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:250

The antibody detects a full length human MuRF1 recombinant protein on SDS-PAGE immunoblots.

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_3068466