Anti-myosine 4/MyHC-IIB (C-terminus) Antilichaam

Non-muscle myosin II is an actin-based motor protein essential to cell motility, cell division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC) . In vertebrates, there are three NMHC-II isoforms (NMHC-IIA, NMHC-IIB, and NMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation of NMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, leading to activation of myosin II motor activity and increased myosin filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin. NMHC-II phosphorylation may be an important mode for regulating myosin-II assembly. PKC phosphorylates NMHC-IIA on Ser-1916 in the C-terminal region and NMHC-IIB on multiple serines in the tailpiece. Casein kinase II phosphorylates NMHC-IIA on Ser-1943 in the tailpiece and increases disassembly of NMHC-IIA filaments.

Our Myosin 4/MyHC-IIB (C-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Myosin 4/MyHC-IIB (C-terminus) and is antigen affinity purified. It is great for use in ELISA, WB.

Our Myosin 4/MyHC-IIB (C-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Myosin 4/MyHC-IIB (C-terminus) and is antigen affinity purified. It is great for use in WB.

Myh4, myosin Iib, myHC-2b; myosin heavy chain 4; MHC2B; Myhsf; MYH2B; AI506973; MyHC IIb

MYH4

Q5SX39

Human, Mouse, and Rat

A synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus from mouse MYH4/MyHC-IIB. This sequence has 100% identity with rat and human MYH4, and has significant homology to MYH1, MYH2, and MYH8.

Myosin 4/MyHC-IIB (C-terminus)

Unconjugated

ELISA, WB

Cell Adhesion Molecules, Cell Signaling, Cytoskeletal Regulation, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

215

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:2000

The antibody detects a 215 kDa* protein corresponding to the apparent molecular mass of MYH4 on SDS-PAGE immunoblots of mouse extraocular muscle and mouse C2C12 cells. The antibody may also detect other skeletal MYHs, such as MYH1 and MYH2, which have significant homology to the C-terminal region of MYH4.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Mouse

Quality Control: WB

AB_3068467