Anti-N4BP1 Antibody Picoband® fluoro594 Vervoeging

Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin–protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73α, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73α for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73α and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.

Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; H3FJ

N4BP1

9683

O75113

Human

No cross-reactivity with other proteins.

E.coli-derived human N4BP1 recombinant protein (Position: M1-K885) .

Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Flow Cytometry

DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling, RNA Processing

Immunogen affinity purified.

Liquid

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

1. Gitlin, A. D., Heger, K., Schubert, A. F., Reja, R., Yan, D., Pham, V. C., Suto, E., Zhang, J., Kwon, Y. C., Freund, E. C., Kang, J., Pham, A., and 13 others. Integration of innate immune signalling by caspase-8 cleavage of N4BP1. Nature 587: 275-280, 2020. 2. Gross, M. B. Personal Communication. Baltimore, Md. 12/23/2020. 3. Murillas, R., Simms, K. S., Hatakeyama, S., Weissman, A. M., Kuehn, M. R. Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase. J. Biol. Chem. 277: 2897-2907, 2002.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Histone cluster 1 H3 family member a/b/c/d/e/f/g/h/i/j

Cytoplasm.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.