Anti-NADPHoxidase 1 NOX1 Antibody Picoband® fluoro488 Vervoeging

NOX1 (NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.

NADPH oxidase 1; NOX-1;1.-.-.-; Mitogenic oxidase 1; MOX-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NOH-1; NOX1; MOX1, NOH1

NOX1

27035

Q9Y5S8

Human

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence in the middle region of human NOX1.

NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH- 1S is detected only in colon and colon carcinoma cells.

Flow Cytometry

Adaptive Immunity, Cancer, Cell Biology, Channels, DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, Immunology, Metabolic Signaling Pathways, Metabolism, Nucleotide Metabolism, Oxidative Stress, Pathways and Processes, Plasma Membrane, Redox Metabolism, Signal Transduction, T Cells

Immunogen affinity purified.

Liquid

NOH-1S is a voltage-gated proton channel that mediates the H (+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H (+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.

1. Banfi, B., Maturana, A., Jaconi, S., Arnaudeau, S., Laforge, T., Sinha, S., Ligeti, E., Demaurex, N., Krause, K.-H. A mammalian H+ channel generated through alternative splicing of the NADPH oxidase homolog NOH-1. Science 287: 138-141, 2000. 2. Behrens, M. M., Ali, S. S., Dao, D. N., Lucero, J., Shekhtman, G., Quick, K. L., Dugan, L. L. Ketamine-induced loss of phenotype of fast-spiking interneurons is mediated by NADPH-oxidase. Science 318: 1645-1647, 2007. 3. Lachgar A, Sojic N, Arbault S, et al. (1999) . Amplification of the inflammatory cellular redox state by human immunodeficiency virus type 1-immunosuppressive tat and gp160 proteins.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

64871 MW

No cross reactivity with other proteins.

6

NADPH oxidase 1

Cell projection, invadopodium membrane ; Multi-pass membrane protein .

Contains 1 FAD-binding FR-type domain.

NADPH oxidase 1 (NOX-1)

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.