Anti-NCX1/SLC8A1 Antibody Picoband® fluoro647 Vervoeging

In cardiac myocytes, Ca (2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca (2+) concentration during contraction is primarily due to release of Ca (2+) from intracellular stores. However, some Ca (2+) also enters the cell through the sarcolemma (plasma membrane) . During relaxation, Ca (2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca (2+) that entered across the sarcolemma must be extruded from the cell. The Na (+) -Ca (2+) exchanger is the primary mechanism by which the Ca (2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.

Transient receptor potential cation channel subfamily V member 3; TrpV3; Vanilloid receptor-like 3; VRL-3; TRPV3

SLC8A1

6546

P32418

Human

No cross-reactivity with other proteins.

E.coli-derived human NCX1/SLC8A1 recombinant protein (Position: E301-Q686) .

Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion. Expressed in the keratinocyte layers of the outer root sheath and, to lesser extent, to the matrix of the hair follicles.

Flow Cytometry

Epigenetics and Nuclear Signaling, Transcription, Cell Biology, Transcription Factors, Cell Cycle

Immunogen affinity purified.

Liquid

Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression.

1. Ebert, A. M., Hume, G. L., Warren, K. S., Cook, N. P., Burns, C. G., Mohideen, M. A., Siegal, G., Yelon, D., Fishman, M. C., Garrity, D. M. Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts. Proc. Nat. Acad. Sci. 102: 17705-17710, 2005. 2. Hilge, M., Aelen, J., Vuister, G. W. Ca (2+) regulation in the Na (+) /Ca (2+) exchanger involves two markedly different Ca (2+) sensors. Molec. Cell 22: 15-25, 2006. 3. Iwamoto, T., Kita, S., Zhang, J., Blaustein, M. P., Arai, Y., Yoshida, S., Wakimoto, K., Komuro, I., Katsuragi, T. Salt-sensitive hypertension is triggered by Ca (2+) entry via Na+/Ca+ exchanger type-1 in vascular smooth muscle. Nature Med. 10: 1193-1199, 2004.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

127459 MW

6

Transient receptor potential cation channel subfamily V member 3

Membrane. Multi-pass membrane protein.

AF4/FMR2 family member 4

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.