Anti-NEBL Antibody Picoband® 31.0550 Vervoeging

Nebulette is a cardiac-specific isoform belonging to the nebulin family of proteins. It is encoded by the NEBL gene. This gene encodes a nebulin like protein that is abundantly expressed in cardiac muscle. The encoded protein binds actin and interacts with thin filaments and Z-line associated proteins in striated muscle. This protein may be involved in cardiac myofibril assembly. A shorter isoform of this protein termed LIM nebulette is expressed in non-muscle cells and may function as a component of focal adhesion complexes. Alternate splicing results in multiple transcript variants.

Protein phosphatase 1E; Ca (2+) /calmodulin-dependent protein kinase phosphatase N; CaMKP-N; CaMKP-nucleus; CaMKN; Partner of PIX 1; Partner of PIX-alpha; Partner of PIXA; PPM1E; CAMKN; KIAA1072; POPX1

NEBL

10529

O76041

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human NEBL recombinant protein (Position: E86-K842) .

Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.

Flow Cytometry

Growth Factors/Hormones, Signal Transduction

Immunogen affinity purified.

Liquid

Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2.

1. Arimura, T., Nakamura, T., Hiroi, S., Satoh, M., Takahashi, M., Ohbuchi, N., Ueda, K., Nouchi, T., Yamaguchi, N., Akai, J., Matsumori, A., Sasayama, S., Kimura, A. Characterization of the human nebulette gene: a polymorphism in an actin-binding motif is associated with nonfamilial idiopathic dilated cardiomyopathy. Hum. Genet. 107: 440-451, 2000. 2. Li, B., Zhuang, L., Trueb, B. Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1. J. Biol. Chem. 279: 20401-20410, 2004. 3. Millevoi, S., Trombitas, K., Kolmerer, B., Kostin, S., Schaper, J., Pelin, K., Granzier, H., Labeit, S. Characterization of nebulette and nebulin and emerging concepts of their roles for vertebrate Z-discs. J. Molec. Biol. 282: 111-123, 1998.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Protein phosphatase, Mg2+/Mn2+ dependent 1E

Nucleus. Cytoplasm.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.