Anti-NEFH-antilichaam (monoclonaal, N52)

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT) .

Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Neurofilament heavy polypeptide; NF-H;200 kDa neurofilament protein; Neurofilament triplet H protein; Nefh; Nfh

NEFH

Human (4744), Mouse (380684), Rat (24587)

P16884

Human, Mouse, Rat

No cross-reactivity with other proteins

C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.

Clone: N52

Muscle specific. Located in the thin filament of striated muscle.

IHC, WB

Alzheimer's Disease, Cell Adhesion Proteins, Cell Type Marker, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction

Ascites

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Lyophilized

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

1. Lees, J. F.; Shneidman, P. S.; Skuntz, S. F.; Carden, M. J.; Lazzarini, R. A. : The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it. EMBO J. 7: 1947-1955, 1988. 2. Brownlees, J.; Ackerley, S.; Grierson, A. J.; Jacobsen, N. J. O.; Shea, K.; Anderton, B. H.; Leigh, P. N.; Shaw, C. E.; Miller, C. C. J. : Charcot-Marie-Tooth disease neurofilament mutations disrupt neurofilament assembly and axonal transport. Hum. Molec. Genet. 11: 2837-2844, 2002.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=MA1071

115378 MW

200 kDa

No cross reactivity with other proteins.

Neurofilament heavy polypeptide

Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.

Belongs to the intermediate filament family.

Neurofilament heavy polypeptide

Mouse IgG1

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.