Anti-Nesprin 2/SYNE2 Antibody Picoband® fluoro488 Vervoeging

Nesprin-2 is a protein that in humans is encoded by the SYNE2 gene. The protein encoded by this gene is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene.

E3 SUMO-protein ligase PIAS3; Protein inhibitor of activated STAT protein 3; PIAS3

SYNE2

23224

Q8WXH0

Human, Monkey

No cross-reactivity with other proteins.

E.coli-derived human Nesprin 2/SYNE2 recombinant protein (Position: Q3765-Q5590) .

Widely expressed.

Flow Cytometry

Cell Biology, Co-Activators/Co-Repressors, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Transcription, Transcription Factors, Ubiquitin & Ubiquitin Like Modifiers

Immunogen affinity purified.

Liquid

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184) . Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032) . Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376) .

1. Apel, E. D., Lewis, R. M., Grady, R. M., Sanes, J. R. Syne-1, a dystrophin- and Klarsicht-related protein associated with synaptic nuclei at the neuromuscular junction. J. Biol. Chem. 275: 31986-31995, 2000. 2. Libotte, T., Zaim, H., Abraham, S., Padmakumar, V. C., Schneider, M., Lu, W., Munck, M., Hutchison, C., Wehnert, M., Fahrenkrog, B., Sauder, U., Aebi, U., Noegel, A. A., Karakesisoglou, I. Lamin A/C-dependent localization of nesprin-2, a giant scaffolder at the nuclear envelope. Molec. Biol. Cell 16: 3411-3424, 2005. 3. Maddox, D. M., Collin, G. B., Ikeda, A., Pratt, C. H., Ikeda, S., Johnson, B. A., Hurd, R. E., Shopland, L. S., Naggert, J. K., Chang, B., Krebs, M. P., Nishina, P. M. A mutation in Syne2 causes early retinal defects in photoreceptors, secondary neurons, and Mueller glia. Invest. Ophthal. Vis. Sci. 56: 3776-3787, 2015.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

96950 MW

6

Protein inhibitor of activated STAT 3

Cytoplasm.

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.